Mainstreaming Real Estate Education on Mandated Courses, Current Strategy and Structure: A Proximity Analysis

This study dived into the importance of mainstreaming real estate education in mandated courses and analyses the current strategies and structures utilized for this purpose. The goal of this proximity analysis was to determine the effectiveness of these strategies and structures in improving the knowledge and skillset of professionals in the field. The study used a mixed-methods approach, combining quantitative and qualitative data from various sources. The findings of this study provide insights into the current state of real estate education in mandated courses and suggest potential improvements to enhance the learning outcomes of professionals in the field. The study recommends the following to mainstream real estate education mandated courses and improve the education system: an online learning platform, more practical and hands-on training, mentorship programs, continuing education and professional development opportunities, and increased emphasis on real estate ethics and standards

Precise Timing of ATPase Activation Drives Targeting of Tail-Anchored Proteins

Hundreds of proteins are anchored on cellular membranes by a transmembrane domain (TM) at their extreme C-terminus. These ‘tail-anchored' (TA) proteins perform numerous essential cellular functions, yet their unique topology poses fundamental challenges to their proper localization. In eukaryotic cells, the highly conserved ATPase Get3 coordinates the efficient capture and delivery of TA proteins to the endoplasmic reticulum (ER). How Get3 uses its ATPase cycles to drive this fundamental process remains elusive. Here we define the Get3 ATPase cycle and show that ATP specifically induces conformational changes in Get3 that culminate in its ATPase activation through tetramerization. This activation is inhibited by the TA protein loading complex Get4/5 and is activated by the TA substrate, ensuring the precise timing of ATP hydrolysis during targeting. Our results provide an explicit model for how Get3 harnesses the energy from ATP to spatially and temporally coordinate the membrane localization of TA proteins

The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle

TEP1 is a protein component of two ribonucleoprotein complexes: vaults and telomerase. The vault-associated small RNA, termed vault RNA (VR), is dependent upon TEP1 for its stable association with vaults, while the association of telomerase RNA with the telomerase complex is independent of TEP1. Both of these small RNAs have been shown to interact with amino acids 1–871 of TEP1 in an indirect yeast three-hybrid assay. To understand the determinants of TEP1–RNA binding, we generated a series of TEP1 deletions and show by yeast three-hybrid assay that the entire Tetrahymena p80 homology region of TEP1 is required for its interaction with both telomerase and VRs. This region is also sufficient to target the protein to the vault particle. Electrophoretic mobility shift assays using the recombinant TEP1 RNA-binding domain (TEP1–RBD) demonstrate that it binds RNA directly, and that telomerase and VRs compete for binding. VR binds weakly to TEP1–RBD in vitro, but mutation of VR sequences predicted to disrupt helices near its central loop enhances binding. Antisense oligonucleotide-directed RNase H digestion of endogenous VR indicates that this region is largely single stranded, suggesting that TEP1 may require access to the VR central loop for efficient binding

Differential gradients of interaction affinities drive efficient targeting and recycling in the GET pathway

Efficient and accurate localization of membrane proteins requires a complex cascade of interactions between protein machineries. This requirement is exemplified in the guided entry of tail-anchored (TA) protein (GET) pathway, where the central targeting factor Get3 must sequentially interact with three distinct binding partners to ensure the delivery of TA proteins to the endoplasmic reticulum (ER) membrane. To understand the molecular principles that provide the vectorial driving force of these interactions, we developed quantitative fluorescence assays to monitor Get3–effector interactions at each stage of targeting. We show that nucleotide and substrate generate differential gradients of interaction energies that drive the ordered interaction of Get3 with successive effectors. These data also provide more molecular details on how the targeting complex is captured and disassembled by the ER receptor and reveal a previously unidentified role for Get4/5 in recycling Get3 from the ER membrane at the end of the targeting reaction. These results provide general insights into how complex protein interaction cascades are coupled to energy inputs in biological systems

Faculty Perception toward Online Education in a State College in the Philippines during the Coronavirus Disease 19 (COVID-19) Pandemic

This research determined the perception toward online education among faculty in a State College in the Philippines. This study used a descriptive online survey involving a sample of 27 faculty members. Statistical tools employed were descriptive statistics, Mann-Whitney U test and Kruskal-Wallis test. Research findings indicated that the majority of faculty had intermediate computer competency and had no training in online teaching with only a few having a very stable internet connection. Faculty considered that online education will result to more academic dishonesty, will be impersonal and lack feeling compared to face-to-face classes, and will be difficult to manage in terms of technology. Additionally, faculty were undecided if they are in favor of online education. The faculty significantly differed whether they are in favor of online education based on age, sex, college, educational attainment, years in teaching, academic rank, level taught and employment status. Faculty of Higher Education Institutions must be provided with continued support and training as they adapt into the new normal in the higher education landscape and as they embrace the instructional challenges brought by the Coronavirus disease 19 pandemic

Filipino Nursing Students' eHealth Literacy and Criteria Used for Selection of Health Websites

Possessing eHealth literacy skills among students has been increasingly appreciated in nursing education. Despite eHealth literacy has been widely studied, little published research is available regarding Filipino nursing students’ eHealth literacy. This study aimed to determine the self-reported eHealth literacy of Filipino nursing students and the relative importance of the criteria they use when selecting health websites. A cross-sectional research design was used involving 66 conveniently chosen nursing students. Self-reported data were collected using adopted questionnaires. Data were analyzed using descriptive statistics, the Mann-Whitney U test, and Spearman’s rho. Findings revealed that nursing students had high eHealth literacy. The utility of information and accuracy of information were the top criteria students consider important when selecting health websites. There were no significant differences in the eHealth literacy of male and female and first-year and second-year nursing students. The extent of Internet use for health information, perceived usefulness of the Internet when making health decisions, and perceived importance to access health resources on the Internet were significantly correlated with eHealth literacy. To derive full benefit from online health information, nursing students’ literacy needs in the Web context and digital environment must be given attention

Complexity Aided Design: the FuturICT Technological Innovation Paradigm

"In the next century, planet earth will don an electronic skin. It will use the Internet as a scaffold to support and transmit its sensations. This skin is already being stitched together. It consists of millions of embedded electronic measuring devices: thermostats, pressure gauges, pollution detectors, cameras, microphones, glucose sensors, EKGs, electroencephalographs. These will probe and monitor cities and endangered species, the atmosphere, our ships, highways and fleets of trucks, our conversations, our bodies--even our dreams ....What will the earth's new skin permit us to feel? How will we use its surges of sensation? For several years--maybe for a decade--there will be no central nervous system to manage this vast signaling network. Certainly there will be no central intelligence...some qualities of self-awareness will emerge once the Net is sensually enhanced. Sensuality is only one force pushing the Net toward intelligence". These statements are quoted by an interview by Cherry Murray, Dean of the Harvard School of Engineering and Applied Sciences and Professor of Physics. It is interesting to outline the timeliness and highly predicting power of these statements. In particular, we would like to point to the relevance of the question "What will the earth's new skin permit us to feel?" to the work we are going to discuss in this paper. There are many additional compelling questions, as for example: "How can the electronic earth's skin be made more resilient?"; "How can the earth's electronic skin be improved to better satisfy the need of our society?";"What can the science of complex systems contribute to this endeavour?

Thermal and cardiovascular strain imposed by motorcycle protective clothing under Australian summer conditions

Motorcycle protective clothing can be uncomfortably hot during summer, and this experiment was designed to evaluate the physiological significance of that burden. Twelve males participated in four, 90-min trials (cycling 30 W) across three environments (25, 30, 35 °C [all 40% relative humidity]). Clothing was modified between full and minimal injury protection. Both ensembles were tested at 25 °C, with only the more protective ensemble investigated at 30 and 35 °C. At 35 °C, auditory canal temperature rose at 0.02 °C min(-1) (SD 0.005), deviating from all other trials (p \u3c 0.05). The thresholds for moderate (\u3e38.5 °C) and profound hyperthermia (\u3e40.0 °C) were predicted to occur within 105 min (SD 20.6) and 180 min (SD 33.0), respectively. Profound hyperthermia might eventuate in ~10 h at 30 °C, but should not occur at 25 °C. These outcomes demonstrate a need to enhance the heat dissipation capabilities of motorcycle clothing designed for summer use in hot climates, but without compromising impact protection. Practitioner\u27s Summary: Motorcycle protective clothing can be uncomfortably hot during summer. This experiment was designed to evaluate the physiological significance of this burden across climatic states. In the heat, moderate (\u3e38.5 °C) and profound hyperthermia (\u3e40.0 °C) were predicted to occur within 105 and 180 min, respectively